Skip Navigation



AOBPreview published online on July 26, 2004
Annals of Botany, doi:10.1093/aob/mch165
© 2004 by Annals of Botany Company
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Select
Right arrow All Versions of this Article:
94/3/449    most recent
mch165v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by CHEN, L.-S.
Right arrow Articles by NOSE, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by CHEN, L.-S.
Right arrow Articles by NOSE, A.
Agricola
Right arrow Articles by CHEN, L.-S.
Right arrow Articles by NOSE, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Submitted on March 19, 2004
Revised on May 16, 2004
Accepted on June 7, 2004

Day-Night Changes of Energy-rich Compounds in Crassulacean Acid Metabolism (CAM) Species Utilizing Hexose and Starch

LI-SONG CHEN1* and AKIHIRO NOSE2

Affiliation of the authors: 1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, P.R. China; 2 Faculty of Agriculture, Saga University, Saga, 840-8502 Japan

* To whom correspondence should be addressed. E-mail: lisongchen2002{at}hotmail.com.

• Background and Aims Plants with crassulacean acid metabolism (CAM) can be divided into two groups according to the major carbohydrates used for malic acid synthesis, either polysaccharide (starch) or monosaccharide (hexose). This is related to the mechanism and affects energy metabolism in the two groups. In Kalanchoë pinnata and K. daigremontiana, which utilize starch, ATP-dependent phosphofructokinase (tonoplast inorganic pyrophosphatase) activity is greater than inorganic pyrophosphate-dependent phosphofructokinase (tonoplast adenosine triphosphatase) activity, but the reverse is the case in pineapple (Ananas comosus) utilizing hexose. To test the hypothesis that the energy metabolism of the two groups differs, day-night changes in the contents of ATP, ADP, AMP, inorganic phosphate (Pi), hosphoenolpyruvate (PEP) and inorganic pyrophosphate (PPi) in K. pinnata and K. daigremontiana leaves and in pineapple chlorenchyma were analysed.

• Methods The contents of energy-rich compounds were measured spectrophotometrically in extracts of tissue sampled in the light and dark, using potted plants, kept for 15 d before the experiments in a growth chamber.

• Key Results In the three species, ATP content and adenylate energy charge (AEC) increased in the dark and decreased in the light, in contrast to ADP and AMP. Changes in ATP and AEC were greater in Kalanchoë leaves than in pineapple chlorenchyma. PPi content in the three species increased in the dark, but on illumination it decreased rapidly and substantially, remaining little changed through the rest of the light period. Pi content of Kalanchoë leaves did not change between dark and light, whereas Pi in pineapple chlorenchyma increased in the dark and decreased in the light, and the changes were far greater than in Kalanchoë leaves. Light-dark changes in PEP content in the three species were similar.

• Conclusions These results corroborate our hypothesis that day-night changes in the contents of energy-rich compounds differ between CAM species and are related to the carbohydrate used for malic acid synthesis.

 Keywords: Ananas comosus, ATP, chlorenchyma, crassulacean acid metabolism, inorganic pyrophosphate, Kalanchoë daigremontiana, Kalanchoë pinnata, phosphoenolpyruvate.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
E. Martinoia, M. Maeshima, and H. E. Neuhaus
Vacuolar transporters and their essential role in plant metabolism
J. Exp. Bot., January 1, 2007; 58(1): 83 - 102.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.