Skip Navigation



AOBPreview published online on March 24, 2005
Annals of Botany, doi:10.1093/aob/mci127
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
95/7/1163    most recent
mci127v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by PHARMAWATI, M.
Right arrow Articles by FINNEGAN, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PHARMAWATI, M.
Right arrow Articles by FINNEGAN, P. M.
Agricola
Right arrow Articles by PHARMAWATI, M.
Right arrow Articles by FINNEGAN, P. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received July 28, 2004
Revised November 22, 2004
Accepted February 9, 2005

Article

Molecular Variation and Fingerprinting of Leucadendron Cultivars (Proteaceae) by ISSR Markers

MADE PHARMAWATI 1*, GUIJUN YAN 1, and PATRICK M. FINNEGAN 1

1 School of Plant Biology, Faculty of Natural and Agricultural Sciences, University of Western Australia, 35 Stirling Highway, Crawley, 6009 WA, Australia

* To whom correspondence should be addressed.
MADE PHARMAWATI, E-mail: pharmawati{at}hotmail.com


  Abstract

• Background and Aims There are more than 80 species of Leucadendron and most are used as cut flowers. Currently, more than 100 cultivars are used by industry and many of them are interspecific hybrids. The origin of most cultivars is unclear and their genetic diversity and relationships have not been studied. This investigation was carried out to evaluate the genetic variation and relationships among 30 Leucadendron cultivars.

• Methods ISSR markers were applied to determine the genetic variation and to discriminate Leucadendron cultivars. Sixty-four ISSR primers were screened and 25 primers were selected for their ability to produce clear and reproducible patterns of multiple bands.

• Key Results A total of 584 bands of 305-2400 bp were amplified, of which 97 % were polymorphic. A dendrogram generated using the Unweighted Pair Group Method with Arithmetic Average based on a distance measure of total character difference showed that the Leucadendron cultivars clustered into two main groups. Twenty-four of the 30 cultivars can be unequivocally differentiated, but identical profiles were observed for three cultivar pairs, ‘Katie's Blush’ and ‘Silvan Red’, ‘Highlights’ and ‘Maui Sunset’, and ‘Yellow Crest’ and ‘Yellow Devil’.

• Conclusions ISSR profiling is a powerful method for the identification and molecular classification of Leucadendron cultivars. A fingerprinting key was generated based on the banding patterns produced using two ISSR primers (UBC856 and UBC857). In addition cultivar-specific ISSR bands were obtained for 17 of the 30 Leucadendron cultivars tested.

Keywords: Fingerprinting key, genetic variation, ISSR, Leucadendron, molecular relationships, Proteaceae.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.