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AOBPreview published online on June 8, 2005
Annals of Botany, doi:10.1093/aob/mci181
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© The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received March 17, 2005
Revised April 7, 2005
Accepted April 28, 2005

Short Communication

Mitochondrial Behaviour in the Early Stages of ROS Stress Leading to Cell Death in Arabidopsis thaliana

KEIKO YOSHINAGA 1, SHIN-ICHI ARIMURA 2, YASUO NIWA 3, NOBUHIRO TSUTSUMI 2, HIROFUMI UCHIMIYA 4, and MAKI KAWAI-YAMADA 4*

1 Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan; Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2 Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
3 Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan
4 Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan

* To whom correspondence should be addressed.
MAKI KAWAI-YAMADA, E-mail: mkawai{at}iam.u-tokyo.ac.jp


  Abstract

• Background and Aims Reactive oxygen species (ROS) are involved in triggering cell death. To visualize mitochondrial behaviour under ROS stress, transgenic arabidopsis plants possessing mitochondrial-targeted GFP (S65T) were studied.

• Methods Arabidopsis leaves were treated with ROS and ROS-inducing chemicals such as hydrogen peroxide, paraquat and menadione. Microscopic observations were carried out using a confocal laser scanning microscope system, and electrolyte leakage was also monitored.

• Key Results After treatment, mitochondria showed morphological changes from a bacillus-like to a round shape. The size of mitochondria treated with H2O2 decreased by half compared with controls. Concurrently, cytoplasmic streaming was blocked and mitochondria eventually swelled. Treatment of leaves with butanedione monoxime, an inhibitor of myosin ATPase, resulted in similar behaviour of mitochondria to that under ROS stress.

• Conclusions The results indicate that morphological changes of mitochondria and cessation of cytoplasmic streaming may interact, and this phenomenon is one of the features of ROS stress-induced cell death.

Keywords: Mitochondria, cytoplasmic streaming, arabidopsis.
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