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AOBPreview published online on November 25, 2005
Annals of Botany, doi:10.1093/aob/mcj024
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© The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received May 19, 2005
Revised July 26, 2005
Accepted October 15, 2005

Short Communication

Genetic Variation in Five Mediterranean Populations of Juniperus phoenicea as Revealed by Inter-Simple Sequence Repeat (ISSR) Markers

MARILENA MELONI 1, DAVIDE PERINI 1, ROSSELLA FILIGHEDDU 2, and GIORGIO BINELLI 1 *

1 Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, via J.H. Dunant 3, 21100 Varese, Italy
2 Dipartimento di Botanica ed Ecologia Vegetale, Via Muroni 25, 07100 Sassari, Italy

* To whom correspondence should be addressed.
GIORGIO BINELLI, E-mail: giorgio.binelli{at}uninsubria.it


  Abstract

• Background and Aims The assessment of the genetic variability and the identification of isolated populations within a given species represent important information to plan conservation strategies on a genetic basis. In this work, the genetic variability in five natural populations of Juniperus phoenicea, three from Sardinia, one from Cyprus and the last one in the Maritime Alps was analysed by means of ISSRs, on the hypothesis that the latter could have been a refugial one during the last glaciation.

• Methods ISSRs were chosen because of their ability to detect variation without any prior sequence information. The use of three primers yielded 45 reproducible, polymorphic bands, which were utilized to estimate the basic parameters of genetic variability and diversity.

• Key Results All of the populations analysed harboured an adequate amount of genetic variability, with HS = 0·1299. The proportion of genetic diversity between populations has been estimated by GST = 0·12. The three Sardinian populations are separated, as tested by AMOVA, from the Cyprus and the continental ones.

• Conclusions The results indicate that geographical isolation has represented a major barrier to gene flow in Juniperus phoenicea. This work represents a first step towards a full genetic characterization of a conifer from the Mediterranean, a world biodiversity hotspot confronted with climate change, and thus contributes towards the planning of genetics-informed conservation strategies.

Keywords: Juniperus phoenicea L, genetic variation, ISSR, conservation, Cupressaceae.
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