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AOBPreview published online on December 12, 2005
Annals of Botany, doi:10.1093/aob/mcj034
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Published by Oxford University Press on behalf of the Annals of Botany Company 2005
Received July 11, 2005
Revised October 12, 2005
Accepted November 6, 2005

Article

Chemical and Histochemical Analysis of ‘Quatre Saisons Blanc Mousseux’, a Moss Rose of the Rosa x damascena Group

JEAN-CLAUDE CAISSARD 1, VÉRONIQUE BERGOUGNOUX 1, MAGALI MARTIN 2, MÉLANIE MAURIAT 1, and SYLVIE BAUDINO 1 *

1 Laboratoire BVpam (Biotechnologies Végétales, plantes aromatiques et médicinales), EA 3061, Université Jean Monnet, 23, rue du Docteur Paul Michelon, 42023 Saint-Etienne cedex 2, France
2 Laboratoire GEPS (Génome et Evolution des Plantes Supérieures), EA 3731, Bâtiment F.A. Forel, Université Claude Bernard Lyon 1, 43 boulevard du 11 novembre 1918, 69622 Villeurbane cedex, France

* To whom correspondence should be addressed.
SYLVIE BAUDINO, E-mail: sylvie.baudino{at}univ-st-etienne.fr


  Abstract

• Background and Aims Moss roses are old garden roses covered with a mossy growth on flower pedicel and calyx. This moss releases a pine-scented oleoresin that is very sticky and odoriferous. Rosa x centifolia ‘muscosa’ was the first moss rose to be obtained by bud-mutation but, interestingly, R. x damascena ‘Quatre Saisons Blanc Mousseux’ was the first repeat-blooming cultivar, thus interesting breeders. In the present study, the anatomy of these sports (i.e. bud-mutations) is characterized and the volatile organic compounds (VOCs) produced by the moss versus the petals are identified. They are compared between the two lines and their respective parents.

• Methods Anatomy of the moss is studied by environmental scanning electron microscopy and histochemical light microscopy. Sudan Red IV and Fluorol Yellow 088 are used to detect lipids, and 1-naphthol reaction with N,N-dimethyl-p-phenylenediamine to detect terpenes (Nadi reaction). Head-space or solid/liquid extraction followed by gas chromatography and mass spectrometry are used to identify VOCs in moss, trichomes and petals.

• Key Results Moss of the two cultivars has the same structure with trichomes on other trichomes but not exactly the same VOCs. These VOCs are specific to the moss, with lots of terpenes. An identical VOC composition is found in leaves but not in petals. They are nearly the same in the moss mutants and in the respective wild types.

• Conclusions Sepals of moss roses and their parents have a specific VOC pattern, different from that of the petals. The moss corresponds to a heterochronic mutation with trichomes developing on other trichomes. Such a mutation has probably appeared twice and independently in the two lines.

Keywords: Rosa x damascena ‘bifera’, Rosa x damascena ‘Quatre Saisons Blanc Mousseux’, Rosa x centifolia, Rosa x centifolia ‘muscosa’, moss roses, glandular trichomes, histochemistry, volatile organic compounds, gas chromatography, sport, terpenoids, benzenoids.
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