Skip Navigation



AOBPreview published online on February 22, 2006
Annals of Botany, doi:10.1093/aob/mcl013
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
97/4/635    most recent
mcl013v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by PÉREZ-COLLAZOS, E.
Right arrow Articles by CATALÁN, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PÉREZ-COLLAZOS, E.
Right arrow Articles by CATALÁN, P.
Agricola
Right arrow Articles by PÉREZ-COLLAZOS, E.
Right arrow Articles by CATALÁN, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received May 26, 2005
Revised September 1, 2005
Accepted December 13, 2005

Article

Palaeopolyploidy, Spatial Structure and Conservation Genetics of the Narrow Steppe Plant Vella pseudocytisus subsp. paui (Vellinae, Cruciferae)

ERNESTO PÉREZ-COLLAZOS 1 and PILAR CATALÁN 1 *

1 Departamento de Agricultura y Economía Agraria, Escuela Politécnica Superior de Huesca, Universidad de Zaragoza, C/Carretera de Cuarte s/n, E-22071 Huesca, Spain

* To whom correspondence should be addressed.
PILAR CATALÁN, E-mail: pcatalan{at}unizar.es


  Abstract

• Background and Aims Vella pseudocytisus subsp. paui (Cruciferae) is a narrow endemic plant to the Teruel province (eastern Spain), which is listed in the National Catalogue of Endangered Species. Two distinct ploidy levels (diploid, 2n = 34, and tetraploid, 2n = 68) have been reported for this taxon that belongs to the core subtribe Vellinae, a western Mediterranean group of shrubby taxa with a chromosome base number of x = 17. Allozyme and AFLP analyses were conducted (a) to test for the ploidy and putative palaeo-allopolyploid origin of this taxon, (b) to explore levels of genetic diversity and spatial structure of its populations, and (c) to address in-situ and ex-situ strategies for its conservation.

• Methods Six populations that covered the entire geographical range of this taxon were sampled and examined for 19 allozyme loci and three AFLP primer pair combinations. In addition, the gametic progenies of five individuals were analysed for two allozyme loci that showed fixed heterozygosity.

• Key Results Multiple banded allozyme profiles for most of the surveyed loci indicated the polyploidy of this taxon. Co-inherited fixed heterozygous patterns were exhibited by the gametophytic tissues of the mother plants. Both allozyme and AFLP markers detected high levels of genetic diversity, and a strong micro-spatial genetic structure was recovered from AFLP phenetic analyses and Mantel correlograms.

• Conclusions Allozyme data support the hypothesis of an allotetraploid origin of Vella pseudocytisus subsp. paui that could be representative of other taxa of the core Vellinae group. AFLP data distinguished three geographically distinct groups with no genetic interaction among them. Allotetraploidy and outcrossing reproduction have probably contributed to maintenance of high levels of genetic variability of the populations, whereas habitat fragmentation may have enhanced the high genetic isolation observed among groups. In-situ microgenetic reserves and a selective sampling of germplasm stocks for ex-situ conservation of this taxon are proposed.

Keywords: AFLP, allotetraploidy, allozymes, conservation, endangered species, genetic diversity, Iberian endemics, Mantel correlograms, palaeopolyploidy, spatial structure, Vella pseudocytisus subsp. paui, Vellinae.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J HeredHome page
C. J. Baskauf and J. M. Burke
Population Genetics of Astragalus bibullatus (Fabaceae) Using AFLPs
J. Hered., July 1, 2009; 100(4): 424 - 431.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.