Skip Navigation



AOBPreview published online on February 15, 2006
Annals of Botany, doi:10.1093/aob/mcl017
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
97/4/541    most recent
mcl017v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by DHAR, M. K.
Right arrow Articles by GILL, B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by DHAR, M. K.
Right arrow Articles by GILL, B. S.
Agricola
Right arrow Articles by DHAR, M. K.
Right arrow Articles by GILL, B. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received August 15, 2004
Revised October 28, 2004
Accepted December 22, 2005

Article

Characterization and Physical Mapping of Ribosomal RNA Gene Families in Plantago

MANOJ K. DHAR 1 *, BERND FRIEBE 2, SANJANA KAUL 1, and BIKRAM S. GILL 2

1 Department of Biotechnology, University of Jammu, Jammu -- 180 006, J & K, India
2 Department of Plant Pathology, Wheat Genetics Resource Center, 4024 Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA

* To whom correspondence should be addressed.
MANOJ K. DHAR, E-mail: manojkdhar{at}rediffmail.com


  Abstract

• Background and Aims The organization of rRNA genes in cultivated Plantago ovata Forsk. and several of its wild allies was analysed to gain insight into the phylogenetic relationships of these species in the genus which includes some 200 species.

• Methods Specific primers were designed to amplify the internal transcribed spacer (ITS1 and ITS2) regions from seven Plantago species and the resulting fragments were cloned and sequenced. Similarly, using specific primers, the 5S rRNA genes from these species were amplified and subsequently cloned. Fluorescence in-situ hybridization (FISH) was used for physical mapping of 5S and 45S ribosomal RNA genes.

• Results The ITS1 region is 19-29 bp longer than the ITS2 in different Plantago species. The 5S rRNA gene-repeating unit varies in length from 289 to 581 bp. Coding regions are highly conserved across species, but the non-transcribed spacers (NTS) do not match any database sequences. The clone from the cultivated species P. ovata was used for physical mapping of these genes by FISH. Four species have one FISH site while three have two FISH sites. In P. lanceolata and P. rhodosperma, the 5S and 45S (18S-5·8S-25S) sites are coupled.

• Conclusions Characterization of 5S and 45S rRNA genes has indicated a possible origin of P. ovata, the only cultivated species of the genus and also the only species with x = 4, from a species belonging to subgenus Psyllium. Based on the studies reported here, P. ovata is closest to P. arenaria, although on the basis of other data the two species have been placed in different subgenera. FISH mapping can be used as an efficient tool to help determine phylogenetic relationships in the genus Plantago and show the interrelationship between P. lanceolata and P. lagopus.

Keywords: Plantago, Psyllium, rRNA genes, internal transcribed spacers, FISH mapping, phylogeny.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.