Skip Navigation



AOBPreview published online on June 11, 2007
Annals of Botany, doi:10.1093/aob/mcm094
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Content Select
Right arrow All Versions of this Article:
100/2/177    most recent
mcm094v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kim, T. D.
Right arrow Articles by Choi, Y. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, T. D.
Right arrow Articles by Choi, Y. E.
Agricola
Right arrow Articles by Kim, T. D.
Right arrow Articles by Choi, Y. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Developmental Plasticity of Glandular Trichomes into Somatic Embryogenesis in Tilia amurensis

T. D. Kim1, B. S. Lee2, T. S. Kim2 and Y. E. Choi1,*

1 Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chunchon 200-701, Korea
2 Forest Seed Research Center, Korea Forest Research Institute, Chungju 380-940, Korea

* For correspondence. E-mail yechoi{at}kangwon.ac.kr

Received: 27 December 2006    Returned for revision: 19 February 2007    Accepted: 26 March 2007   

Background and Aims: In Tilia amurensis, two types of trichomes (hairy and glandular) develop from epidermal surfaces of cotyledons and hypocotyls of zygotic embryos soon after germination. Here, it is demonstrated that glandular trichome initials develop directly into somatic embryos when treated in vitro with 2,4-dichlorophenoxyacetic acid (2,4-D).

Methods: Zygotic embryos of Tilia amurensis were cultured on Murashige and Skoog medium with 3 % sucrose and various concentrations (0, 2·2, 4·4 and 8·8 µM) of 2,4-D. Morphological development of trichomes and somatic embryos was analysed by scanning electron microscope and light microscope after histological sectioning.

Key Results: In zygotic embryos cultured on medium with 4·4 µM 2,4-D, formation of hairy trichomes was completely suppressed but formation of glandular trichome initials increased. That some filamentous trichome initials developed directly into somatic embryos was confirmed by histological and scanning electron microscope observation. When explants with different stages of trichome initials (two-, four- and eight-celled filamentous and fully mature trichomes) were temporally pre-treated with 4·4 µM 2,4-D for 24 h and transferred into hormone-free medium, two-celled and four-celled filamentous trichome initials were the effective stage of trichomes for somatic embryo induction.

Conclusions: It is suggested that early developing filamentous trichome initials have developmental plasticity and that with 2,4-D treatment these trichome initials develop directly into somatic embryos.

Key words: Tilia amurensis, glandular trichome, hairy trichome, somatic embryo, filamentous trichome initials, developmental plasticity


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.