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AOBPreview published online on February 15, 2008
Annals of Botany, doi:10.1093/aob/mcn016
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© The Author 2008. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping

J. King1,*, D. Thorogood1, K. J. Edwards2, I. P. Armstead, L. Roberts1, K. Skøt1, Z. Hanley3 and I. P. King1

1 Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, SY23 3EB, UK
2 School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK
3 Vialactia Biosciences, PO Box 109-185, Newmarket, Auckland, New Zealand

* For correspondence. E-mail julie.king{at}bbsrc.ac.uk

Received: 25 May 2007    Returned for revision: 25 July 2007    Accepted: 11 January 2008   

Background and Aims: Perennial ryegrass (Lolium perenne) is one of the key forage and amenity grasses throughout the world. In the UK it accounts for 70 % of all agricultural land use with an estimated farm gate value of £6 billion per annum. However, in terms of the genetic resources available, L. perenne has lagged behind other major crops in Poaceae. The aim of this project was therefore the construction of a microsatellite-enriched genomic library for L. perenne to increase the number of genetic markers available for both marker-assisted selection in breeding programmes and gene isolation.

Methods: Primers for 229 non-redundant microsatellite markers were designed and used to screen two L. perenne genotypes, one amenity and one forage. Of the 229 microsatellites, 95 were found to show polymorphism between amenity and forage genotypes. A selection of microsatellite primers was selected from these 95 and used to screen two mapping populations derived from intercrossing and backcrossing the two forage and amenity grass genotypes.

Key Results and Conclusions: The utility of the resulting genetic maps for analysis of the genetic control of target traits was demonstrated by the mapping of genes associated with heading date to linkage groups 4 and 7.

Key words: Microsatellites, Lolium perenne, perennial ryegrass, trait mapping


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