AOBPreview published online on September 2, 2008
Annals of Botany, doi:10.1093/aob/mcn150
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Morphology of Nectaries and Biology of Nectar Production in the Distylous Species Fagopyrum esculentum
1 Groupe de Recherche en Physiologie Végétale – Unité de Biologie Végétale, Université catholique de Louvain, Croix du Sud 5, boîte 13, B-1348 Louvain-la-Neuve, Belgium
2 Groupe de Recherches ‘Génétique, Reproduction, Populations’ – Unité de Génétique et Centre de Recherche sur la Biodiversité, Université catholique de Louvain, Croix du Sud 2, boîte 14, B-1348 Louvain-la-Neuve, Belgium
* For correspondence. E-mail anne-laure.jacquemart{at}uclouvain.be
Received: 11 March 2008 Returned for revision: 28 April 2008 Accepted: 8 July 2008
Background and Aims: The mechanisms of floral nectar production in buckwheat (Fagopyrum esculentum, Polygonaceae), a distylous pseudo-cereal, have received relatively little attention, prompting an investigation of the factors that regulate this process. The aim was to perform a refined study of the structures that secrete nectar and of the internal and external parameters influencing nectar volumes and sugar concentrations.
Methods: In order to control environmental parameters, plants were cultivated in growth rooms under controlled conditions. The structure of nectaries was studied based on histological sections from flowers and flower buds. Nectar was extracted using glass micropipettes and the sugar concentration was measured with a hand refractometer. Sugar concentration in the phloem sap was measured using the anthrone method. To test the influence of photosynthesis on nectar production, different light and defoliation treatments were applied.
Key Results: Unicellular trichomes were located in the epidermis at the ventral part of eight nectary glands situated on the flower receptacle alternately with stamens. Vascular bundles consisting of both phloem and xylem were identified at the boundary between a multilayered nectary parenchyma and a sub-nectary parenchyma with chloroplasts. A higher volume of nectar in thrum morphs was observed. No other difference was found in morphology or in sugar supply to inflorescences between morphs. Nectar secretion was strongly influenced by plant age and inflorescence position. Nectar volumes were higher in the upper inflorescences and during the flowering peak. Light had a dual role, (1) acting directly on reproductive structures to trigger flower opening, which conditions nectar secretion, and (2) stimulating photosynthetic activity, which regulates nectar accumulation in open flowers.
Conclusions: In buckwheat, nectar is secreted by trichomes and probably proceeds, at least in part, from phloem sap. Nectar secretion is strongly influenced by floral morph type, plant age, inflorescence position and light.
Key words: Buckwheat, distyly, Fagopyrum esculentum, inflorescence position, morph comparisons, nectary histology, nectar sugar concentration, nectar volume, light intensity, organ biomass, phloem sap, plant age