Skip Navigation



AOBPreview published online on January 19, 2009
Annals of Botany, doi:10.1093/aob/mcn268
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
103/5/749    most recent
mcn268v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Schuette, S.
Right arrow Articles by Renzaglia, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schuette, S.
Right arrow Articles by Renzaglia, K. S.
Agricola
Right arrow Articles by Schuette, S.
Right arrow Articles by Renzaglia, K. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Novel localization of callose in the spores of Physcomitrella patens and phylogenomics of the callose synthase gene family

Scott Schuette1,*, Andrew J. Wood1, Matt Geisler1, Jane Geisler-Lee1, Roberto Ligrone2 and Karen S. Renzaglia1

1 Department of Plant Biology – 6509, Southern Illinois University, Carbondale, IL 62901, USA
2 Dipartimento di Scienze ambientali, Seconda Università di Napoli, via Antonio Vivaldi 43 81100 Caserta, Italy

* For correspondence. E-mail renzlab{at}siu.edu

Received: 12 October 2008    Returned for revision: 4 November 2008    Accepted: 25 November 2008   

Background and Aims: Callose involvement in spore development is a plesiomorphic feature of land plants. Correlated light, fluorescence and immuno-electron microscopy was conducted on the developing spores of Physcomitrella patens to probe for callose. Using a bioinformatic approach, the callose synthase (PpCalS) genes were annotated and PpCalS and AtCalS gene families compared, testing the hypothesis that an exine development orthologue is present in P. patens based on deduced polypeptide similarity with AtCalS5, a known exine development gene.

Methods: Spores were stained with aniline blue fluorescent dye. Capsules were prepared for immuno-light and immuno-electron microscopy by gold labelling callose epitopes with monoclonal antibody. BLAST searches were conducted using the AtCalS5 sequence as a query against the P. patens genome. Phylogenomic analysis of the CalS gene family was conducted using PAUP (v.4·1b10).

Key Results: Callose is briefly present in the aperture of developing P. patens spores. The PpCalS gene family consists of 12 copies that fall into three distinct clades with AtCalS genes. PpCalS5 is an orthologue to AtCalS5 with highly conserved domains and 64 % similarity of their deduced polypeptides.

Conclusions: This is the first study to identify the presence of callose in moss spores. AtCalS5 was previously shown to be involved in pollen exine development, thus making PpCalS5 a suspect gene involved in moss spore exine development.

Key words: Bryophyte, callose, callose synthase, exine development, moss, Physcomitrella patens, spores, sporogenesis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
P. Apostolakos, P. Livanos, T. L. Nikolakopoulou, and B. Galatis
The role of callose in guard-cell wall differentiation and stomatal pore formation in the fern Asplenium nidus
Ann. Bot., December 1, 2009; 104(7): 1373 - 1387.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.