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AOBPreview published online on June 30, 2009
Annals of Botany, doi:10.1093/aob/mcp150
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© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Sucrose importation into laticifers of Hevea brasiliensis, in relation to ethylene stimulation of latex production

Anaïs Dusotoit-Coucaud1, Nicole Brunel1, Panida Kongsawadworakul2, Unchera Viboonjun2, André Lacointe1, Jean-Louis Julien1, Hervé Chrestin2,3 and Soulaïman Sakr1,4,*

1 UMR 547 PIAF, INRA, Université Blaise Pascal, 24 avenue des Landais, 63177 Aubière Cedex, France
2 Department of Plant Science, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
3 Institut de Recherche pour le Developpement (IRD), UR 060 CLIFA/CEFE-CNRS, 1919 route de Mende, F34293, Montpellier Cedex 5, France
4 Agrocampus Ouest, Centre d'Angers, UMR SAGAH, IFR QUASAV 149, 2 rue le Nôtre, 49045 Angers Cedex, France

* For correspondence. E-mail soulaiman.sakr{at}agrocampus-ouest.fr

Received: 2 February 2009    Returned for revision: 31 March 2009    Accepted: 29 April 2009   

Background and Aims: The major economic product of Hevea brasiliensis is a rubber-containing cytoplasm (latex), which flows out of laticifers (latex cells) when the bark is tapped. The latex yield is stimulated by ethylene. Sucrose, the unique precursor of rubber synthesis, must cross the plasma membrane through specific sucrose transporters before being metabolized in the laticifers. The relative importance of sucrose transporters in determining latex yield is unknown. Here, the effects of ethylene (by application of Ethrel®) on sucrose transporter gene expression in the inner bark tissues and latex cells of H. brasiliensis are described.

Methods: Experiments, including cloning sucrose transporters, real time RT-PCR and in situ hybridization, were carried out on virgin (untapped) trees, treated or untreated with the latex yield stimulant Ethrel.

Key Results: Seven putative full-length cDNAs of sucrose transporters were cloned from a latex-specific cDNA library. These transporters belong to all SUT (sucrose transporter) groups and differ by their basal gene expression in latex and inner soft bark, with a predominance of HbSUT1A and HbSUT1B. Of these sucrose transporters, only HbSUT1A and HbSUT2A were distinctly increased by ethylene. Moreover, this increase was shown to be specific to laticifers and to ethylene application.

Conclusion: The data and all previous information on sucrose transport show that HbSUT1A and HbSUT2A are related to the increase in sucrose import into laticifers, required for the stimulation of latex yield by ethylene in virgin trees.

Key words: Hevea brasiliensis, laticifers, latex production, ethylene, sucrose transporters


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K. Tungngoen, P. Kongsawadworakul, U. Viboonjun, M. Katsuhara, N. Brunel, S. Sakr, J. Narangajavana, and H. Chrestin
Involvement of HbPIP2;1 and HbTIP1;1 Aquaporins in Ethylene Stimulation of Latex Yield through Regulation of Water Exchanges between Inner Liber and Latex Cells in Hevea brasiliensis
Plant Physiology, October 1, 2009; 151(2): 843 - 856.
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